quant kit Search Results


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Primers for real-time <t> RT-qPCR. </t>
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New England Biolabs nebnext library quant kit for illumina
Primers for real-time <t> RT-qPCR. </t>
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Image Search Results


Primers for real-time  RT-qPCR.

Journal: Oncology Reports

Article Title: Promoting roles of the secreted frizzled-related protein 2 as a Wnt agonist in lung cancer cells

doi: 10.3892/or.2015.4221

Figure Lengend Snippet: Primers for real-time RT-qPCR.

Article Snippet: For RT-qPCR, 2 µ g of total RNA and primers were mixed with regents supplied by Quant One Step RT-qPCR (SYBR Green I) kit (Tiangen, Beijing, China) according to the instructions.

Techniques:

The SFRP2 expression profiles in lung cancer cell lines. (A) Real-time RT-qPCR detection of SFRP2 mRNA levels in the lung cancer cell lines 95-D, A549 and SPCA1. (B) Representative images for western blotting of SFRP2 protein in 95-D, A549 and SPCA1 cells. Data are presented as mean ± SD. Statistical significance was evaluated using the Student's t-test. ** P-value ≤0.01.

Journal: Oncology Reports

Article Title: Promoting roles of the secreted frizzled-related protein 2 as a Wnt agonist in lung cancer cells

doi: 10.3892/or.2015.4221

Figure Lengend Snippet: The SFRP2 expression profiles in lung cancer cell lines. (A) Real-time RT-qPCR detection of SFRP2 mRNA levels in the lung cancer cell lines 95-D, A549 and SPCA1. (B) Representative images for western blotting of SFRP2 protein in 95-D, A549 and SPCA1 cells. Data are presented as mean ± SD. Statistical significance was evaluated using the Student's t-test. ** P-value ≤0.01.

Article Snippet: For RT-qPCR, 2 µ g of total RNA and primers were mixed with regents supplied by Quant One Step RT-qPCR (SYBR Green I) kit (Tiangen, Beijing, China) according to the instructions.

Techniques: Expressing, Quantitative RT-PCR, Western Blot